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KMID : 1098420020100040294
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Korean Journal of Medicinal Crop Science
2002 Volume.10 No. 4 p.294 ~ p.297
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Cloning and expression of glutathione S-transferase(GST) cDNA from Gossypium hirsutum L.
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Kang, Won Hee
Kim, Myong Jo/Lim, Jung Dae/Yun, Song Joong/Chung, Ill Min/Yu, Chang Yeon
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Abstract
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A gene coding for the GST of cotton (Gh-5) was cloned into Escherichia coli and expressed. The enzyme remained within the cytoplasm of E. coli. An 696 bp open reading frame was in the 988 base pair fragment of the recombination plasmid pET-30b(+). The deduced protein sequence consists of 232 amino acids and has a molecular mass of 30235.58 Da. The cloned enzyme conjugated reduced glutathione and 1-chloro-2, 4-dinitrobenzene (CDNB). Plant GST cDNA was expressed in microbe and produced polypeptide had function as an enzyme.
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